Microscopy

Microscopy

Electronical Microscopy

Preparation of biological samples for MET observation:

• Fixation, dehydration and inclusion for ultrastructural analysis
• immunocytochemical techniques with labelling to colloidal gold in pre or post coating,
• Cryo-techniques, cryo-substitution, cryo-cuts,
• Negative staining of macromolecules and cellular fractions
• Ultrathin sections and cryo-sections followed contrast

Preparation of biological samples for SEM observation:

• Metallisation and desiccation by bypassing critical point

Optical microscopy (photonics)

• Visualization and localization of fluorescent molecules in confocal microscopy and video microscopy
• Study of the dynamics of migration and subcellular compartments.
• Spectral analysis to autofluorescence
• Multidimensional acquisition field video microscopy to track events on live samples,
• Three-dimensional reconstruction, quantification (fluorescence, counting, pattern recognition, etc.)
• Mosaic
• Processing and Image Analysis (Fiji, Imaris)